INDICATORS ON GENETIC METHYLATION TESTING YOU SHOULD KNOW

treatment method with sodium bisulfite deaminates unmethylated cytosine to uracil, whereas 5mC will not be impacted. Subsequent PCR amplification will replace the uracils by thymines. Reads from NGS libraries produced in parallel with and without bisulfite treatment are mapped on to reference genomes and also the contrasting C/T positions concernin